What is Cellobiase?
Cellobiase (also known as 𝛽-glucosidase) is an enzyme, specifically part of the cellulase family, that catalyzes the breakdown of cellobiose into two molecules of glucose. It is crucial in cellulose hydrolysis, preventing feedback inhibition of other cellulases (endoglucanase/exoglucanase). Cellobiase is essential for producing biofuels (cellulosic ethanol) and in food processing, such as reducing bitterness in fruits.
Everything You Need to Know
What is cellobiase used for?
Cellobiase is an enzyme which can hydrolyze the substrate with β- glucosidic bond. It can be used in the alcohol fermentation. It can hydrolyze the β-D-glucosidic bond of reducing end, which the acquisitions are isomalto-oligosaccharide, sugar ester, glycopeptide and etc.
Does cellobiase convert glucose to cellulose?
Cellobiase can hydrolyze cellobiose into glucose; it plays a key role in the process of cellulose hydrolysis by reducing the product inhibition. To reuse the enzyme and improve the economic value of cellulosic ethanol, cellobiase was immobilized using sodium alginate and chitosan as carriers by the bubbling method.
Is cellobiose safe to consume?
Literature data as well as a human tolerance study performed by the applicant indicated the tolerable dose of cellobiose consumption to be 20 g cellobiose daily for single-use and 15 g twice daily (30 g total) for repeated consumption.
What are the 4 types of enzymes?
There are six main categories of enzymes: oxidoreductases, transferases, hydrolases, lyases, isomerases, and ligases.
Main Details:
Physical Appearance: Typically ranges from pale yellow to light brown, with color intensity varying slightly based on purity, manufacturing processes, or added stabilizers. Higher purity samples tend to be paler.
Source: It can be derived from various organisms, including fungi (e.g., Aspergillus, Trichoderma), bacteria, and certain plants, through fermentation and purification processes.
Stability: As a powder, it generally has better storage stability compared to liquid formulations, often retaining activity for extended periods when stored in cool, dry conditions.
Industrial Applications: Used in, but not limited to, the food-processing industry for flavoring and aroma enhancement, and in the production of bioethanol.
Immobilization: It can be immobilized using agents like chitosan-alginate, improving its stability and efficiency in industrial applications.
Role in Hydrolysis: It breaks down cellobiose ( 𝛽 -D-glucose bond) to release 𝛽 -D-glucose, completing the degradation of cellulose into glucose.
ANALYTICAL RESULTS
| Test Item | Method / Ref. | Specification | Result | Conclusion |
|---|---|---|---|---|
| Identification | In-house ID / label claim | Cellobiase (β-glucosidase) | Conforms | Pass |
| Appearance | Visual | White to off-white powder | Off-white powder | Pass |
| Enzyme Activity | β-glucosidase assay / supplier method | NLT 5,000 U/g | 5,200 U/g | Pass |
| Activity Unit Definition | Supplier-defined unit | Report only | U/g reported | Pass |
| pH (1% solution) | pH meter | 4.5 – 6.5 | 5.1 | Pass |
| Loss on Drying | Gravimetric / USP 731 | ≤ 8.0% | 4.3% | Pass |
| Lead (Pb) | ICP-MS / ICP-OES | ≤ 5.0 mg/kg | 0.18 mg/kg | Pass |
| Arsenic (As) | ICP-MS / ICP-OES | ≤ 3.0 mg/kg | 0.09 mg/kg | Pass |
| Cadmium (Cd) | ICP-MS / ICP-OES | ≤ 0.5 mg/kg | < 0.05 mg/kg | Pass |
| Mercury (Hg) | ICP-MS / ICP-OES | ≤ 0.5 mg/kg | < 0.01 mg/kg | Pass |
| Total Plate Count | Plate count | ≤ 50,000 CFU/g | 1.9 × 10³ CFU/g | Pass |
| Coliforms | Plate count | ≤ 30 CFU/g | < 10 CFU/g | Pass |
| E. coli | Absence Test | Negative / 25g | Not Detected | Pass |
| Salmonella | Absence Test | Negative / 25g | Not Detected | Pass |
Production Process
