Animal protein hydrolysates (APH) are high-quality, bioactive protein sources created by breaking down animal by-products (meat, fish, collagen, intestines) using enzymatic hydrolysis. They provide essential amino acids, improve digestibility, and enhance palatability, making them ideal for young livestock, pet food, and specialized animal diets.
Source Material: Raw materials often include nutrient-rich by-products from slaughterhouses or fish processing, including cartilage, bones, blood, skin, viscera, and chicken feathers.
Everything You Need to Know
Can humans eat hydrolyzed protein?
The FDA has approved hydrolyzed soy protein as a food ingredient. However, no studies are proving that hydrolyzed soy protein has any health benefits over other types of protein powder or meal replacement shakes. Hydrolyzed soy protein is derived from a process that breaks down soy protein into its amino acids.
What is protein hydrolase?
Protein hydrolysis is the process of breaking down proteins into their constituent amino acids through the addition of water molecules. Enzymes, specialised proteins that act as biological catalysts, facilitate this process.
What are the side effects of hydrolyzed beef protein?
While Hydrolyzed Beef Protein Isolate is generally safe for consumption, it is always best to consult your physician before adding any new supplements or nutrition products to your diet. Some individuals may experience digestive issues such as bloating, gas, or stomach discomfort when consuming protein supplements.
What are the disadvantages of hydrolyzed protein?
The adverse effects of hydrolyzed protein include anxiety, asthma, attention deficit syndrome, bloating, burning sensations, carpel tunnel syndrome, chest pains, depression, diarrhea, confusion, dizziness, drowsiness, infertility, insomnia, and heart diseases (Scopp, 1991).
Main Details:
Applications in Nutrition:
Pet Food: Known for creating savory tastes (umami) and high palatability for cat and dog food.
Livestock & Aquaculture: Enhances growth performance and feed efficiency in young animals like pigs, calves, and fish
Functionality: Improves digestibility and reduces allergenicity in specialized diets
Production Process: Enzymes such as endo-proteases are used to cut proteins into smaller, highly soluble, and digestible peptides. This is often preferred over chemical methods as it is a controlled, "soft" process that preserves amino acid bioavailability.
Benefits:
Improved Nutrition: High-quality amino acid profile and increased solubility.
Sustainability: Reduces waste by converting by-products into high-value nutrition.
Health: Can reduce allergenicity and support gut health.
Side Effects: Can lead to poor palatability (pets may refuse to eat it) and, in some cases, cause diarrhea or vomiting due to high osmotic pressure.
ANALYTICAL RESULTS
| Test Item | Specification | Result | Method |
|---|---|---|---|
| Appearance | Light yellow to yellow-brown powder | Conforms | Visual |
| Odor | Characteristic savory/protein odor | Conforms | Organoleptic |
| Solubility | Partially to freely soluble in water | Conforms | In-house |
| Crude Protein | ≥ 80.0% | 86.4% | Kjeldahl / AOAC |
| Degree of Hydrolysis | 15.0–25.0% | 19.8% | OPA / In-house validated |
| Moisture | ≤ 8.0% | 5.3% | Loss on Drying |
| Ash | ≤ 12.0% | 7.6% | AOAC / Muffle Furnace |
| Fat | ≤ 6.0% | 2.4% | Soxhlet / AOAC |
| pH (10% solution) | 5.5–7.5 | 6.4 | pH Meter |
| Total Nitrogen | ≥ 12.0% | 13.8% | Kjeldahl |
| Peptide Content | ≥ 70.0% | 78.5% | In-house |
| Lead (Pb) | ≤ 1.0 mg/kg | 0.12 mg/kg | ICP-MS |
| Cadmium (Cd) | ≤ 0.5 mg/kg | < 0.05 mg/kg | ICP-MS |
| Arsenic (As) | ≤ 1.0 mg/kg | 0.09 mg/kg | ICP-MS |
| Mercury (Hg) | ≤ 0.1 mg/kg | < 0.01 mg/kg | ICP-MS |
| Total Plate Count | ≤ 10,000 CFU/g | 320 CFU/g | ISO 4833 / USP <61> |
| Yeast & Mold | ≤ 100 CFU/g | < 10 CFU/g | ISO 21527 / USP <61> |
| Coliforms | ≤ 10 CFU/g | < 10 CFU/g | ISO 4832 |
| Escherichia coli | Negative / g | Not Detected | USP <62> / ISO 16649 |
| Salmonella | Negative / 25 g | Not Detected | USP <62> / ISO 6579 |
| Staphylococcus aureus | Negative / g | Not Detected | USP <62> |
| Particle Size (80 mesh) | ≥ 95.0% pass | 98.2% | Sieve Analysis |
Production Process
