Inulinase, also known as inulase, is a highly efficient hydrolytic enzyme that specifically catalyzes the breakdown of inulin (a polyfructan carbohydrate) into fructose, glucose, and fructooligosaccharides (FOS). As a green biocatalyst, it is widely used in food, feed, bioenergy, functional health products and other industries, with the advantages of high specificity, mild reaction conditions and environmental friendliness.
This product is prepared via advanced microbial fermentation technology, featuring stable enzyme activity, strong hydrolysis efficiency and wide adaptability, fully complying with international food and feed safety standards, and suitable for large-scale industrial production and application.
Everything You Need to Know
What is the enzyme in inulin?
What are the sources of inulinase?
Many microorganisms, including fungi, yeasts, and bacteria, are known to produce inulinase. The biochemical properties of enzymes depend most of all on their source. Inulinase production has been reported from various sources, such as animals, stored inulin tissues of plants, and multiple microorganisms
Is inulin the same as inulinase?
Inulinases are part of the glycoside hydrolase family (GH32) that catalyzes the hydrolysis of inulin, a polydisperse fructan composed of β-2,1-linked d-fructose molecules with a terminal glycosyl residue, and of inulin-type fructans.
Which is better, inulin or psyllium?
Inulin, a soluble and fermentable fiber, has shown potential in promoting weight loss and reducing triacylglycerol (TAG) levels in individuals with obesity and dyslipidemia [20,21,22]. Psyllium, a viscous, gel-forming fiber, is noted for its lesser fermentation, thereby reducing gastrointestinal discomfort.
Main Details:
Functions
Studies have reported that inulinase can act not only on inulin, but also on sucrose and raffinose, and shows higher vitality and hydrolysis capacity. Using Jerusalem artichoke extract or inulin as a carbon source can induce inulinase production, but Jerusalem artichoke extract is better as a substrate. This may be because the extract contains more short-chain polyfructose in addition to inulin, which is more conducive to the growth of the bacteria and hydrolysis by enzymes. That is to say, a certain substrate can induce the production of inulinase, but the use of different carbon sources as substrates has a great influence on the activity of the enzyme. Studies have found that a certain concentration of inulin, maltose and low concentration of fructose can induce the production of inulinase, starch has little effect on inulinase, but glucose can significantly inhibit inulinase activity. It shows that inulinase has substrate specificity.
Applications
Production of high fructose syrup by inulinase Because high fructose syrup is cheap, sweet, refreshing, high in osmotic pressure, good preservation effect, low calorific value, not easy to cause dental caries, and can be used by diabetics, so in the United States, Japan and other developed countries It is widely used in the food and pharmaceutical industries. After the 1970s, countries began to focus on the preparation of fructose by using inulin as a raw material and acid and enzymatic hydrolysis. Although the acid method has high output, it has many by-products, heavy pigments, and is difficult to separate and refine. In the 1980s, researchers from the United States, the French, Bristol-Myers, Canada and other countries began to study the use of inulinase to make fructose. Its process is simple, the conversion rate is high, the product is pure, and the fructose output is high. It can directly produce ultra-high fructose syrup (uhfgs), with fructose content above 90%. To this end, developed countries such as the United States, Britain, Denmark, France, and Canada are conducting research. This shows that inulinase has great potential for development and application in the production of fructose and fructose syrup. Exo-inulinase degradation products are mainly fructose and the proportion of fructose is high. There are many studies on the production of high fructose syrup by inulinase at home and abroad, and China's research is still in its infancy.
ANALYTICAL RESULTS
| Test Item | Specification | Method | Result | Conclusion |
|---|---|---|---|---|
| Appearance | Off-white to light yellow powder | Visual | Off-white powder | Conforms |
| Odor | Characteristic | Organoleptic | Characteristic | Conforms |
| Inulinase Activity | ≥ 10,000 U/g | Internal Method ENZ-IM-021 | 12,480 U/g | Conforms |
| Loss on Drying | ≤ 8.0% | USP <731> / 105°C | 4.6% | Conforms |
| pH (1% solution) | 4.0 – 6.5 | USP <791> | 5.2 | Conforms |
| Ash | ≤ 8.0% | USP <281> | 3.1% | Conforms |
| Lead (Pb) | ≤ 3.0 mg/kg | ICP-MS | 0.21 mg/kg | Conforms |
| Arsenic (As) | ≤ 2.0 mg/kg | ICP-MS | 0.09 mg/kg | Conforms |
| Cadmium (Cd) | ≤ 1.0 mg/kg | ICP-MS | 0.04 mg/kg | Conforms |
| Mercury (Hg) | ≤ 0.1 mg/kg | ICP-MS | < 0.01 mg/kg | Conforms |
| Total Plate Count | ≤ 10,000 CFU/g | USP <61> | 320 CFU/g | Conforms |
| Yeast & Mold | ≤ 100 CFU/g | USP <61> | 18 CFU/g | Conforms |
| Coliforms | ≤ 10 CFU/g | USP <62> | < 10 CFU/g | Conforms |
| Escherichia coli | Negative / g | USP <62> | Negative | Conforms |
| Salmonella | Negative / 25 g | USP <62> | Negative | Conforms |
Production Process
