What is Alkaline Protease?
Alkaline protease is a highly active industrial enzyme (often from Bacillus strains) that hydrolyzes protein peptide bonds at an optimal pH of 9–11. It is the most demanded enzyme, crucial for detergent, leather, food, and pharmaceutical industries due to its stability in high pH. It acts by breaking down complex proteins into amino acids.
Alkaline protease production typically involves microbial fermentation and optimized processes, aiming for high-efficiency, sustainable industrial applications.
Everything You Need to Know
What does alkaline protease do?
Alkaline protease refers to an enzyme that can hydrolyze protein peptide bonds under alkaline conditions, and the optimum pH is in the range of 9-11. It is widely used in detergent, food, medical, brewing, silk, tanning and other industries.
What are the three proteases?
Like all proteases, pepsin breaks down proteins into peptides. It is one of three proteases in the human digestive system, along with trypsin and chymotrypsin. Pepsin is involved in the first phase of protein digestion.
Can protease help with inflammation?
Levene in his studies on "The Cleavage Products of Proteases" in the first issue of The Journal of Biological Chemistry published October 1, 1905, and used successfully in Germany in the 1960s, it was discovered that the recognized benefits included: reducing inflammation, decreasing pain from osteoarthritis, treating ...
What is alkaline and its benefits?
Alkaline water has a higher pH than that of plain tap water. So proponents say that alkaline water can neutralize acid in the body. Some say that alkaline water can help prevent disease, such as cancer and stroke. But more research is needed to prove these claims.
Main Details:
Uses:
Detergents: The largest application, breaking down protein-based stains (blood, grass, food).
Leather Industry: Used in dehairing and bating (softening) skins, replacing toxic chemicals.
Food Industry: Applied in protein hydrolysis, such as creating fish protein hydrolysates and modifying food textures.
Medical/Biotechnology: Used for debridement in wound care and in laboratory tools like protease K for nucleic acid purification.
Textiles: Used to process silk and wool fabrics.
Applications
• Application of alkaline protease in soybean polypeptide and soybean phospholipid functional food
The soy protein (isolated soy protein or soy protein concentrate, etc.) is hydrolyzed into soy peptides to improve the solubility and functional properties of soy protein. It can be used as a protein supplement and nutritional supplement for beverages, dairy products, and food for the elderly and infants.
• Application of alkaline protease in leather industry
Alkaline protease can be used to make depilatory agent. The tanned leather will be depilated cleanly, with clear grain, no obvious damage, and fine and bright pores.
• Application of alkaline protease in extraction of pharmaceutical raw materials
Alkaline protease can be used for the extraction of heparin and chondroitin sulfate.
ANALYTICAL RESULTS
| Test Item | Method / Ref. | Specification | Result | Conclusion |
|---|---|---|---|---|
| Identification | In-house ID / label claim | Alkaline Protease | Conforms | Pass |
| Appearance | Visual | Light yellow fine powder | Light yellow fine powder | Pass |
| Enzyme Activity | Casein hydrolysis assay / supplier method | NLT 200,000 U/g | 210,000 U/g | Pass |
| Activity Unit Definition | Supplier unit definition | Report only | U/g reported | Pass |
| Odor | Organoleptic | No offensive odor | Conforms | Pass |
| pH (1% solution) | pH meter | 7.0 – 11.0 | 8.9 | Pass |
| Loss on Drying | Gravimetric | ≤ 5.0% | 2.2% | Pass |
| Loss on Ignition / Ash | Gravimetric | ≤ 5.0% | 2.3% | Pass |
| Lead (Pb) | ICP-MS / ICP-OES | ≤ 5.0 mg/kg | < 5.0 mg/kg | Pass |
| Arsenic (As) | ICP-MS / ICP-OES | ≤ 3.0 mg/kg | < 3.0 mg/kg | Pass |
| Heavy Metals (as Pb) | Limit test | ≤ 30 mg/kg | Conforms | Pass |
| Total Plate Count | Plate count | ≤ 10,000 CFU/g | 1,000 CFU/g | Pass |
| Yeast & Mold | Plate count | ≤ 100 CFU/g | Conforms | Pass |
| E. coli | Absence test | None detected | Absent | Pass |
| Salmonella | Absence test | None detected | Absent | Pass |
Production Process
