What is Acid Protease?
Acid proteases (or acid proteinases) are enzymes that catalyze the breakdown of proteins into smaller peptides and amino acids, operating optimally in acidic environments (pH 3–4). Primarily categorized as aspartic proteases, they are vital for industrial food processing, alcoholic beverage production, and digestion
Everything You Need to Know
What is the use of acid protease?
The Role of Acid Protease in Alcohol Fermentation - Enzymes - Angelyeast. Annzyme® acid protease refined by fermentation of Aspergillus niger can effectively hydrolyze protein at low PH, and is widely used in alcohol, liquor, beer, brewing, food processing, feed, leather processing and other industries.
Who should not take protease?
Some may cause allergic reactions. See specific proteolytic enzymes for more detailed safety information. Pregnancy and breast-feeding: There isn't enough reliable information to know if proteolytic enzymes are safe to use when pregnant or breast-feeding. Stay on the safe side and avoid use.
What diseases are associated with proteases?
Several lines of evidence have suggested that proteases participate in the pathogenesis of varieties of diseases, such as cancer, inflammation , neurodegenerative diseases, liver diseases, chronic kidney disease and cardiovascular disease
What is the function of protease?
Thus, proteases regulate the fate, localization, and activity of many proteins, modulate protein-protein interactions, create new bioactive molecules, contribute to the processing of cellular information, and generate, transduce, and amplify molecular signals.
Main Details:
Acid protease means that the protease has a lower optimum pH, rather than the acidic group present in the active part of the enzyme. The optimum pH of the acid protease ranges from about 2 (pepsin) to about 4. From the analysis of enzyme activity-PH curve, there are one or more carboxyl groups in the active part of the enzyme. The most thoroughly studied of this type of protease is pepsin.
Natural protease inhibitors include the alicyclic protein family, which play a role in cell regulation and differentiation. It has been found that lipophilic ligands bound to lipocalin have properties of inhibiting tumor proteases. Do not confuse natural protease inhibitors with protease inhibitors used in antiretroviral therapy. Some viruses, including HIV/AIDS, rely on proteases during their reproductive cycle. Therefore, protease inhibitors have been developed as antiviral means.
ANALYTICAL RESULTS
| Test Item | Method / Ref. | Specification | Result | Conclusion |
|---|---|---|---|---|
| Identification | In-house ID / label claim | Acid Protease | Conforms | Pass |
| Appearance | Visual | Light brown to tan powder | Light brown powder | Pass |
| Enzyme Activity | Casein hydrolysis assay / supplier method | NLT 100,000 U/g | 112,500 U/g | Pass |
| Activity Unit Definition | Supplier unit definition | Report only | U/g reported | Pass |
| Solubility | Water solubility test | Soluble in water | Conforms | Pass |
| pH (1% solution) | pH meter | 2.5 – 6.0 | 4.1 | Pass |
| Loss on Drying | Gravimetric | ≤ 8.0% | 4.7% | Pass |
| Lead (Pb) | ICP-MS / ICP-OES | ≤ 2.0 ppm | 0.18 ppm | Pass |
| Arsenic (As) | ICP-MS / ICP-OES | ≤ 1.0 ppm | 0.09 ppm | Pass |
| Total Plate Count | Plate count | ≤ 50,000 CFU/g | 2.4 × 10³ CFU/g | Pass |
| Coliforms | Plate count | ≤ 30 CFU/g | < 10 CFU/g | Pass |
| E. coli | Absence test | Negative / 25 g | Not Detected | Pass |
| Salmonella | Absence test | Negative / 25 g | Not Detected | Pass |
Production Process
