What is Mannanase?
𝛽-Mannanase is an enzyme that catalyzes the hydrolysis of 𝛽-1,4-d-mannopyranosyl linkages, breaking down complex mannan-based polysaccharides into simple sugars. It is used to improve carbohydrate digestibility in animal feed, reduce viscosity in food processing (like coffee and oil), improve paper pulp bleaching, and remove stains in detergents.
Everything You Need to Know
What is mannanase in laundry detergent?
Mannanase enzymes attack mannan stains by cutting mannan molecules into tiny sugars that dissolve in the wash water. Detergent can now lift the mannan stains away from the fabric.
What is the function of Mannanase?
Several studies have shown that mannanase can maintain intestinal epithelium morphology, improve the digestibility of nutrients (Ko et al., 2021), inhibit the expression of various pathogen-associated signaling pathways (Arsenault et al., 2017a) and alleviate intestinal inflammation (Arsenault et al., 2017b; Ferreira ...
What are the benefits of Mannanase?
Dietary β-mannanase, which is an exogenous enzyme that hydrolyzes β-mannan, has been reported to improve poultry productivity by increasing energy and nutrient utilization via decreasing anti-nutritional effects of β-mannan including increased digesta viscosity
What does mannanase break down?
Mannanase is an enzyme that breaks down mannan polymers, which are hemicelluloses composed of mannose sugars. Mannans are found in the cell walls of many plants, typically comprising a significant percentage of total biomass.
Main Details:
Mannanase occurs throughout the natural world, but microbes are the primary tool through which it is made for commercial purposes. Producing microbial mannanases occurs mostly by submerging bacteria in a liquid that is continuously agitated, or via solid-state fermentation, which involves growing the microbes on a substrate. The microbes secrete the lipase, which is then purified, homogenized, and crystallized for sale
Function: It breaks down mannan, a component of hemicellulose found in plant cell walls, into manno-oligosaccharides.
Applications:
Animal Feed: Improves nutrient absorption in poultry and pigs.
Detergents: Efficiently removes stains from foods like ice cream and sauces.
Pulp & Paper: Used in bleaching to reduce chlorine consumption.
Oil/Gas: Breaks down guar gum in hydraulic fracturing fluids.
ANALYTICAL RESULTS
| Test Item | Method / Ref. | Specification | Result | Conclusion |
|---|---|---|---|---|
| Identification | In-house ID / label claim | Mannanase / β-Mannanase | Conforms | Pass |
| Appearance | Visual | Off-white to light tan powder | Light tan powder | Pass |
| Enzyme Activity | Supplier β-mannanase assay | NLT 10,000 U/g | 10,850 U/g | Pass |
| Activity Unit Definition | Supplier-defined unit | Report only | U/g reported | Pass |
| Solubility | Water solubility test | Soluble / dispersible in water | Conforms | Pass |
| pH (1% solution) | pH meter | 4.0 – 7.0 | 5.6 | Pass |
| Loss on Drying | Gravimetric | ≤ 8.0% | 4.4% | Pass |
| Lead (Pb) | ICP-MS / ICP-OES | ≤ 5.0 mg/kg | 0.22 mg/kg | Pass |
| Arsenic (As) | ICP-MS / ICP-OES | ≤ 3.0 mg/kg | 0.11 mg/kg | Pass |
| Cadmium (Cd) | ICP-MS / ICP-OES | ≤ 0.5 mg/kg | < 0.05 mg/kg | Pass |
| Mercury (Hg) | ICP-MS / ICP-OES | ≤ 0.5 mg/kg | < 0.01 mg/kg | Pass |
| Total Plate Count | Plate count | ≤ 50,000 CFU/g | 2.0 × 10³ CFU/g | Pass |
| Coliforms | Plate count | ≤ 30 CFU/g | < 10 CFU/g | Pass |
| E. coli | Absence test | Negative / 25 g | Not Detected | Pass |
| Salmonella | Absence test | Negative / 25 g | Not Detected | Pass |
Production Process
